Culture of Mycobacterium avium subspecies paratuberculosis from the blood of patients with Crohn\u27s disease

Background Crohn\u27s disease, a form of inflammatory bowel disease, resembles some aspects of tuberculosis, leprosy, and paratuberculosis. The role of Mycobacterium avium subspecies paratuberculosis (MAP) in Crohn\u27s disease is controversial. Methods We tested for MAP by PCR and culture in buffy coat preparations from 28 individuals with Crohn\u27s disease, nine with ulcerative colitis, and 15 without inflammatory bowel disease. Findings MAP DNA in uncultured buffy coats was identified by PCR in 13 (46%) individuals with Crohn\u27s disease, four (45%) with ulcerative colitis, and three (20%) without inflammatory bowel disease. Viable MAP was cultured from the blood of 14 (50%) patients with Crohn\u27s disease, two (22%) with ulcerative colitis, and none of the individuals without inflammatory bowel disease. Current use of immunosuppressive medication did not correlate with a positive MAP culture. Sequencing of PCR products from MAP cultures confirmed the presence of the MAP-specific IS900 fragment. Among 11 MAP isolates assessed, we identified nine strains that were not identical. Interpretation We detected viable MAP in peripheral blood in a higher proportion of individuals with Crohn\u27s disease than in controls. These data contribute to the evidence that MAP might be a cause of Crohn\u27s disease