Pathological study of alcoholicplantagolanceolata crude extracts effectonsome Salmonella species invitroand invivo

This study was designed to explore the pathological effect of alcoholicplantagolanceolata crude extracts effect on some Salmonella species invitro and invivo study by using laboratory mice.plantagolanceolata crude extractswere dealing inin vitro study against somevirulent bacteriaincluding Salmonella typhimurium,Salmonella typhiand Salmonella hadar. And using the concentrations (100,150,200,250 and 300) mgfor plantagolanceolata crude extract,concentration at 300 mghad the highest inhibitory effect on the three types of salmonella,when compared with the other concentrations.The pathologicalstudy ofplantagolanceolata crude extractwas done against the infection of Salmonella typhimuriumas in vivo study by using white laboratory mice. Twenty four mice were randomly divided into six groups, each ghroup contain four animals. First group was administrated orally o.3 ml of Salmonella typhimuriumof bacterial suspension containing 1x106cfu orally for one week of infection. Second group administrated orally with 0.3 ml of bacterial suspension containing 1x106cfu orally of Salmonella typhimuriumfor two weeks as infection. Third group administrated orally with 0.3 ml of bacterial suspension containing 1x106cfu orally Salmonella typhimuriumfor three weeks as infection. Fourth group was administrated orally with o.3 ml of plantagolanceolata extract for one week daily after infection with Salmonella typhimurium. Fifth group was administrated orally with o.3 ml ofplantagolanceolata extract for two weeks daily after infection with Salmonella typhimurium. And sixth group was administrated orally with o.3 ml ofplantagolanceolata extract for three weeks daily after infection with Salmonella typhimurium.The histopathological study showed pathological changes in theintestine of the first , second and third groups that infected with Salmonella typhimuriumbacteria, (sixfhgroup)no clear pathological changes were reported except some lesions. For all, the plantagolanceolata extract apparently has therapeutic effect whenused in high concentration invitro and for long time invivo