Detection of a closteroviruslike particle from a corky bark-affected grapevine cultivar

Closteroviruslike particles were mechanically transmitted from in vitro shoot tip cultures of a grapevine affected with corky bark disease to seedlings of Nicotiana benthamiana. Local necrotic lesions appeared 9-12 d and systemic symptoms 14-16 d post-inoculation. This virus had a normal length of 725 nm, striations with a pitch of 3.4 nm and was 10 nm wide. In ISEM tests, the virus was not decorated with antibodies prepared against GVA, NY-1, CA-4, GLRV-1, GLRV-3, ASGV, PVT or CLSV