Discrimination of wild grapes native to China by RAPD markers

A set of 73 types of 18 wild grape species native to China, one interspecific hybrid, 7 Vitis vinifera cultivars, the rootstock cultivar SO 4 and one V. riparia accession were investigated using the RAPD technique. The screening of 280 decamer oligonucleotides allowed the selection of 20 primers used for the analysis. RAPD fingerprints of 83 grape samples were obtained. 191 bands, intense and easy to score, were chosen as markers. On average 5.7 bands per primer were amplified with an average of 68.7 % polymorphism. The size of amplified bands ranged from 100 to 3000 bp. Discrimination of 83 samples was obtained with one of two primers (OPQ04, OPJ07) combined with one of 5 primers (OPJ01, OPH19, OPP02, OPA15, OPU16). The average number of bands for each sample per primer was 5.7. This revealed a high level of polymorphism among the wild grapes native to China. RAPD markers proved to be useful for identification as they are quick and easy to use.