The influence of Harmonia axyridis morbidity on 2-Isopropyl-3-methoxypyrazine in 'Cabernet Sauvignon' wine

Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae; the Multicolored Asian Lady Beetle; MALB) is a vineyard pest in many winemaking regions of the world due to its ability to taint juice and wine (‘ladybug taint’) through excretion or extraction of 2-isopropyl-3-methoxypyrazine (IPMP) when the beetles are incorporated with the grapes at harvest. A common vineyard intervention is the use of insecticidal sprays and the resulting dead beetles are often incorporated in with the harvested fruit. The main objective of this study was to quantify the impact of dead MALB on IPMP concentrations in red wine. Duplicate 'Cabernet Sauvignon' wines were produced with the addition of 10 beetles·l-1 juice, added either live or at 1, 3, 7, or 60-days postmortem. A control wine with no added beetles was included. IPMP concentration was substantially higher in live-beetle wines, and decreased to base-line levels at approximately 6.5 days post-mortem. These results should assist in decisions on viticultural interventions, such as timing of insecticidal sprays, as well as the establishment of grape quality parameters.