Preparation and Standardisation of Ayurvedic Polyherbal Formulation: Patoladi Kvatha Churna for Skin Diseases

Raw materials standardisation was done to confirm the absence of adulterants in the crude drugs and thereby it ensures a quality product. Experiments for all physicochemical parameters were performed for both the raw materials as well as the finished products as per API and results obtained were verified with the limits and proved to be successful. An extensive toxicological evaluation was done using the latest equipments to ensure the safety profile of the formulation. Total bacterial count and total fungal count was almost nil. This showed that the formulation is free of pathogenic organisms. The levels of heavy metals, pesticides, Aflatoxins were also determined. The results obtained were appreciable. Estimation of sodium content in the formulation revealed that the percentage of sodium is within the specified limits. Qualitative and quantitative phytochemical screening was performed for the raw materials as well as the formulation. It was found to contain almost all the major Phytoconstituents. TLC studies were performed for individual crude drug. The Rf values of formulation are compared with the Rf value of crude drug, thereby ensuring the presence of the individual herb in the formulation. GC-MS analysis was done to identify the presence of various chemical structures with the help of the peaks obtained. HPTLC studies were also done to get the finger printing of the formulation. Quercetin used as bio marker. Finger printing shows the excellent presence of quercetin in the PKC. In vitro anti-bacterial and anti-fungal activity was performed by Disc Diffusion and Well Diffusion method. Since five of the raw material contains flavanoidal content which was revealed by preliminary phytochemical screening and HPTLC studies. The formulation posses an excellent anti-bacterial and anti-fungal activity. Thus an Indian traditional official Ayurvedic formulation “PATOLADI KVATHA CHURNA” has been standardised Pharcognostically, phytochemical and even pharmacologically. These studies will be helpful to increase the confidence among the people to use this formulation for various disorders