Neuropharmacological Assessment of Desmodium Gangeticum in Partial Sciatic Nerve Ligation in Rat Model: Role of Inflammatory Mediators

The present study was a model to evaluate spatial learning and memory (118). It consisted of large circular pool (150 cm in diameter, 45 cm in height, filled to a depth of 30 cm with water maintained at 28±1oC). During the MWM test, a platform 15 cm in diameter was located 1.5 cm below the water in one of four sections of the pool, approximately 50 cm from the sidewalls. The pool was divided into four quadrants of equal area. A camera was mounted to the ceiling above the pool and the paths taken by the rats were recorded. SUMMARY & CONCLUSION: The present study aimed to evaluate the aqueous extract of Desmodium gangeticum for its protective effect in neuropathic pain activity by using in vitro and in vivo models like LPS induced inflammation in murine macrophages and PSNL model to induce neuropathic pain in rats. We observed the pain and memory functions condition of animals by inducing neuropathic pain using PSNL model in rats. To elucidate the mechanism of action protective effect in neuropathic pain, pro-inflammatory mediators were measured. To support these studies histopathological studies is carried out. PSNL rats showed that significant decrease in pain threshold in pain behaviour studies such as thermal, mechanical allodynia and hyperalgesia and reduced motor coordination and cognitive impairment functions. Aqueous extract of Desmodium gangeticum was prepared by water decoction process. Quantification through HPTLC studies revealed that Desmodium gangeticum contained 0.608mg/g amounts of Quercetin. Desmodium gangeticum exhibited dose dependent protection in cell viability in LPS induced inflammation in murine macrophages. The aqueous extract of Desmodium gangeticum show significant increase in pain threshold in pain behaviour studies such as thermal, mechanical allodynia and hyperalgesia. The aqueous extract of Desmodium gangeticum improved motor coordination and cognitive impairment. The aqueous extract of DG showed significant decrease in pro-inflammatory mediators like TNF-α, IL- 1β, IL-6, COX-2 which shows that anti-inflammatory mechanism of Desmodium gangeticum. The study can be concluded that treatment of Desmodium gangeticum in PSNL induced rat reduced mechanical and thermal hyperalgesia and allodynia. It also improved the long term spatial memory and recognition memory. They exert their activity by inhibiting pro inflammatory mediator such as TNF-α, IL- 1β, IL-6, and COX-2 in PSNL affected nerves. The outcome of the study is that the aqueous extract of Desmodium gangeticum may be a potential therapeutic usage as novel anti nociceptive and cognitive impairment therapy in chronic neuropathic condition