Comparison of Remineralization Efficacy on Tooth with Various Remineralizing Agents using Polarized Light Microscopy: An Invitro Study

The aim of this study was to utilize polarized light microscope for the assessment of the efficacy of CPP-ACP, NovaMin and Amine Fluoride pastes on remineralization of enamel over time. 40 teeth were used for the study which was divided into 4 groups: Group A (n=10): Recaldent (casein phosphopeptide-amorphous calcium phosphate) was used as a remineralizing agent. Group B (n=10): NovaMin (Calcium Sodium Phosphosilicate) was used as a remineralizing agent. Group C (n=10): Amine fluoride was used as a remineralizing agent. Group D (n=10): The teeth served as a control in which only demineralization was done. Following one week of demineralization and three weeks of remineralization, the teeth were mounted on cold cure acrylic and the coronal portions were sectioned labiolingually by means of water cooled hard tissue microtome to obtain vertical slices of 400μm thickness. These sections were then used to observe areas of enamel demineralization under a Labomed CXR3 microscope with crossed polarizer and analyzer plates for areas of demineralization. Photography was done using a Nikon Coolpix 4500 digital camera at 2x zoom under a 4X10 x magnification. The depth of demineralization was measured using the IMAGETOOL© software. After assessing different areas, the maximum depth of demineralization was recorded The values obtained were statistically analysed using Student’s t-test to test the difference between the groups, with the p value set at < 0.5 and found that CCP-ACP and Amine fluoride was better in remineralization followed by NovaMin