Effects on Angiogenesis, TNF- α Inhibition of the Leaves of Justicia gendarussa Burm. f

This study covers the pharmacognostic parameters of the leaves of J.gendarussa such as macroscopical, microscopical including powder analysis, quantitative microscopy and physical standards like ash values, extractive values, etc., have been studied and presented. The tissue arrangement in the leaf was studied and presented. The presence of transcurrent palisade in the lamina and the presence of lithocysts with calciumcarbonate bodies. Funnel shaped glandular trichomes. The prelimnary phytochemcial studies revealed the presence of carbohydrate, proteins and aminoacids, glycoside, saponin and terpenoids. HPTLC profiles of ethanolic and petroleum ether extracts were studied and presented. The Significant Antiangiogenic activity at 50μg/ml concentration by both ethanolic and aqueous extracts was studied. The inhibition of neavascularization is concentration dependant. In the cytotoxicity study, CTC-50% of the ethanolic extract was found to be 156.50 μg/ml. The TNF-α inhibitory activity at 100μg/ml (56%) was studied. The inhibition of TNF-α activity is concentration dependant. The antimicrobial activity shows activity against bacillus ------------- and moderate activity against The present study provide scientific basis for the ethanomedical use of this plant in chronic rheumatism and various inflammatory conditions. It is concluded that it can be optimistic that the present work proved that the leaves of J.gendarussa having therapeutic advantage to be a potential phytochemcial target in the design of a single herbal drug molecule for the treatment treatment of angiogenesis dependant diseases without cytotoxic effects with multiple benefits