Studies on the Leaves of Solanum Melongena Var.Melongena and its Promising Ace Inhibition for Various Therapeutic Approaches

This study covers the Pharmacognostic parameters of the leaves of Wrightia tinctoria belonging to the Family Apocynaceae such as macroscopical, microscopical including powder analysis and physical standards like ash value, extractive values etc have been studied and presented. The tissue arrangement in the leaf was studied and presented. Specific microscopical characters of the leaf were as follows: Ground tissue contains wide circular cells which are latricifers or latex secreting cells which are random in distribution without any inclusions. In the venation pattern vein terminals are invariantly forked repeatedly forming dendroid configuration. Calcium oxalate druses abundant under Polarized Light Microscope (50 μm in diameter). The preliminary phytochemical studies in leaf powder revealed the presence of carbohydrate, phytosterols, alkaloids, glycosides, protein and amino acids, terpenoids, and flavanoids. Isolation and identification of β amyrin from the petroleum ether extract. HPTLC of the ethanolic and ethyl acetate extracts were studied and presented. Antimutagenic Study: A simple, economical, reliable laboratory scale Wing Spot Assay on D.melanogaster to assess the mutagenic effect was standardized and presented. In the present study it was confirmed that the ethanolic extract is found to be nonmutagenic (5–50 μg/ml) on D.melanogaster. It need further advanced genotoxic experiments in animal model using SMART assay. (Somatic mutation and Recombination Test Assay). Invitro Dental Plaque Biofilm Inhibitory Activity: The results of the current investigation revealed that the biofilm (Plaque) formation by the S.mutans was inhibited by the ethanolic extract was concentration dependent. There was nil growth of S.mutans observed at 100 and 150 mg/ml concentration. So we were also able to demonstrate significant invitro dental plaque biofilm inhibitory activity was exhibited by the ethanolic extract of the leaves of W.tinctoria against Primary Colonizer Bacterium S.mutans. Moreover this study provides the scientific basis for the Ethnomedical use of this plant as dental analgesic