Effect of polylactic-co-gylcolic acid nanoparticles in photoactivated disinfection of root canals against enterococcus faecalis biofilm: An In vitro study

BACKGROUND: The influence of PLGA nanoparticles in enhancing the photoactivated disinfection of root canals. Aim & Objective: The objective of the study was to evaluate the invitro effects Polylactic-co- glycolic acid (PLGA) nanoparticles loaded with the Photosensitiser Methylene blue (MB) and light against E. faecalis biofilm. MATERIALS AND METHODS: 50 extracted mandibular premolar teeth were decoronated and chemomechanically prepared. Biofilm was allowed to be formed in the root canals of specimens by incubating it for 2 weeks in BHI broth infected with E faecalis. Based on the mode of treatment, the specimens were divided into 5 groups (n=10): Group I- MB encapsulated with PLGA nanoparticles (MB+NP), Group II – MB with passive ultrasonic activation (MB+US), Group III- MB with GP activation (MB+GP), Group IV – MB without any activation and Group V- specimens without any treatment (control). The specimens were incubated with the Photosensitiser for 10 mins and subjected to Photoactivated disinfection using Diode laser (665nm), with a power density of 100mW/cm2 and total energy fluence of 30 J/cm2. The groups were subdivided, 8 specimens were subjected to microbiological analysis and 2 specimens were analysed under CLSM to evaluate the alive/dead bacteria. RESULTS: The MB+NP group showed least number of CFU’s after PAD, and was followed by MB+US group. The control group exhibited maximum number of CFU’s. All the groups showed significant difference when compared to the control group. The CLSM images mirrored and justified the results obtained by microbiological analysis. CONCLUSION: The ultilisation of PLGA nanoparticles encapsulated with photosensitive dye proved to be effective against E faecalis biofilm and can be a promising adjunct in antimicrobial endodontic therapy