Phytochemical Characterisation and in Vitro Anticancer Screeening of Ethanol Extract of Chromolaena Odorata Linn.

The present work is deal with the Phytochemical studies : Collection and authentication of plant material. Solvent extraction of plant material with petroleum ether (60-80 °C). Solvent extraction of plant material with ethanol (95% v/v). Preliminary phytochemical screening of both extracts. Thin layer chromatography of the extracts. Isolation of plant constituent by column chromatography. Characterization of isolated compound by spectral studies. Pharmacological studies: In vitro studies: Evaluation of anticancer activity of the ethanol extract by MTT assay using human colorectal adenocarcinoma cell line (HCT116) and human cervical cancer cell lines (HeLa) from literature survey, preliminary phytochemical screening, thin layer chromatography study, isolation of active compound by column chromatography and spectral studies of the isolated compound by UV, IR and NMR spectroscopy, it reveals that flavonoids may be the secondary metabolite present in active crude extract of leaves of Chromolaena odorata linn which may be responsible for pharmacological activity in the line anticancer activity. Considering the lack and need of drugs, the specific effects of Chromolaena odorata reported in the present study is of enormous interest and deserves further investigation using more experimental paradigms for further confirmation to isolate and characterize the active compound