Method Dvelopment and Validation for the Simultaneous Estimation of Atazanavir Sulphate and Ritonavir in Bulk and Tablet Dosage form by RP-HPLC.

Analytical chemistry may be defined as the science and art of determining the composition of material in terms of elements or compounds contained in it1. Analytical chemistry is divided into two branches quantitative and qualitative. A qualitative method is the information about the identity of atomic or molecular species or functional groups in the sample. A quantitative method provides numerical information as to the relative amount of one or more of these components. For analysis of these drugs different analytical methods are routinely being used. These analytical methods are classified as chemical and instrumental. The chemical methods include Gravimetric and Titrimetric. These methods are simple but less precise and more time consuming so now days these methods are not suggested for the routine analysis. The instrumental methods include electrochemical methods - Electro gravimetric method, Potentiometry, Colorimetry, Conductometry, Polarography and Optical methods - Absorption and Emission methods. The Absorption methods include Visible Spectrophotometry, Ultraviolet Spectrophotometry, Atomic Absorption Spectrophotometry, Infrared Spectrophotometry, and Emission methods include Emission Spectroscopy, Flame Photometry, Fluorimetry, etc. The other outstanding methods include isotopes, radioactivity, X-ray fluorescence and separation methods as various chromatographic principles viz. HPLC, GC, HPTLC, LC-MS. The present study indicated that the developed method is simple, precise and cost effective for the simultaneous estimation of Atazanavir sulphate and Ritonavir for routine quality control analysis of these either in bulk and pharmaceutical formulation. The developed and validated RP-HPLC method outlined is very obvious, affordable, dynamic, low cost, rapid easy to perform with small sample volume and good repeatability. It can be adopted for the routine quality control analysis of simultaneous determination of Atazanavir sulphate and Ritonavir because of good resolution of the chromatographic peaks