Method Dvelopment and Validation for the Simultaneous Estimation of Clidinium Bromide and Chlordizepoxide in Bulk and Tablet Dosage form by a RPHPLC.

The modern form of column chromatography has been called high performance, high pressure, and high-resolution and high-speed liquid chromatography. High-Performance Liquid Chromatography (HPLC) is a special branch of column chromatography in which the mobile phase is forced through the column at high speed. As a result the analysis time is reduced by 1-2 orders of magnitude relative to classical column chromatography and the use of much smaller particles of the adsorbent or support becomes possible increasing the column efficiency substantially. In the present study, new RP-HPLC method for simultaneous estimation of Clidinium bromide and Chlordiazepoxide in bulk and pharmaceutical dosage form was developed. The developed method was validation for various parameters such as accuracy, precision, ruggedness, linearity, robustness, system suitability, specificity, limit of detection and limit of quantification as per ICH guidelines. The developed RP-HPLC method has been successfully applied for the simultaneous determination of Clidinium bromide and Chlordiazepoxide in pure drug and marketed formulation. The methods are found to be rapid, simple, accurate and convenient to adopt. The developed methods are completely validated with all validation parameters.The results indicate that this method are precise, accurate further more it is easy and convenient for routine drug analysis