Evaluation of Hepatoprotective Activity of Ethanolic Extract of Polygonum Glabrum Willd on Hepatotoxicity Induced Rats.

The present study was aimed to assess the hepatoprotective activity of methanolic extract of Polygonum glabrum Willd. LD50 studies were conducted in albino rats with methanolic extract of polygonum glabrum according to OECD guidelines 423 and was found safe upto the dose level of 2000 mg/kg confirming its non-toxic nature. The hepatoprotective activity was studied in antitubercular drugs induced hepatoxic animal models. The physical parameters such as liver weight and biochemical parameters like SGOT, SGPT, ALP, Total protein and Bilirubin. Antioxidant parameters like catalase, GSH and LPO and histopathology reports of livers were also considered to confirm hepatoprotection. Antitubercular drugs induced hepatotoxicity was significantly prevented by pretreatment with methanolic extract of Polygonum glabrum Willd. Decrease in wet liver weight and reduction in biochemical parameters levels like serum SGOT, SGPT, ALP, Total protein bilirubin and increase in ROS scavenging enzyme activities such as catalase GSH and LPO after treatment with methanolic extract of Polygonum glabrum Willd confirmed the hepatoprotective effect of ectract under study. In liver injury models in rats restoration of hepatic cells with minor fatty changes and absence of necrosis after treatment with extract was observed indicating satisfactory hepatoprotection. Based on improvement in serum marker enzyme levels, physical parameter and antioxidant parameters and histopathological studies it was concluded that methanolic extract of Polygonum glabrum possesses significant hepatoprotective activity in the doses used