Comparitive evaluation of loop mediated isothermal amplification in the diagnosis of mycobacterium tuberculosis with culture and fluorescent microscopy.

TB is a common disease and leading causes of mortality due to communicable diseases. Research has been going to find the nature of the disease as well to find new technique that could give us a leading edge in cracking this disease in an early stage.recently a number of nucleic acid amplification assays have been employed to detect the mycobacterium tuberculosis in clinical specimens, among these loop mediated isothermal amplification posess superior reaction characteristics in the present study we evaluate a LAMP method to identify the MTB specific IS6110 gene among 50 sputum samples collected from patients suspected of tuberculosis in tirunelveli medical college and compare them with gold standard “culture on lowenstein jenson media” for 8 weeks .the study showed the LAMP method has an excellent sensitivity of 95% and specificity of 79% which shows that this method in conjunction with smear microscopy can be a powerful tool in the early detection of TB