Evaluation of Methanolic Extract of Hypericum Mysorense Ointment for its Wound Healing Activity

The majority of the populations in the developing world rely on traditional medicine for their primary healthcare needs. Herbal therapy predominates in traditional medicine as well as in complementary or alternative medicine practiced in the developed world. Among the various indications where traditional herbal medicines are used, skin and skin related disorders are ranked top. Thus, the main objective of the present study is to formulate and evaluate semi-solid dosage forms of Hypericum mysorense for its wound healing activity. The stem of Hypericum mysorense was extracted by continuous hot percolation/ soxhletation using methanol as solvent and was evaluated for its phytochemical property, in-vitro antimicrobial and in-vitro antioxidant activity. Using this methanolic extract, semi-solid dosage form(ointment) was formulated. Ointment was prepared using 2% concentration of the extract by fusion method using emulsifying ointment as base and then tested for physiochemical properties like colour, loss on drying, pH, viscosity, melting point, spreadability and extrudability. HPTLC analysis, in-vitro diffusion studies, kinetic studies and stability studies were also conducted on the preparation. The results of in-vitro antimicrobial and in-vitro antioxidant activity added on to the wound healing activity of the extract. Hence, the ointment containing 2% methanolic extract of Hypericum mysorense was evaluated for its wound healing activity using excision wound model. The formulation did not produce any skin irritation for about a week when applied over the skin. The effect produced by the extract ointment, in terms of wound contracting ability, wound closure time and histopathological characteristics were comparable to that of a standard drug, povidone iodine ointment. Therefore, the present study concluded that the Hypericum mysorense at 2% concentration of the methanolic extract formulated as an ointment showed a better wound healing formulation for better patient care and pharmaco economical