Integrated approach of experimental data and computer modeling and simulation for understanding chromatin structure and dynamics

X-ray crystallographic analysis and single molecule analysis of electron microscopy provide us precise images of biomolecules, but the information is basically static. X-ray or neutron scattering give us structural information of the molecules in solution, but they are low resolution. We integrate such information with aids of computer modeling and simulation to build the 3D structures in solution and investigate the dynamics of mono- or di-nucleosome which are fundamental structural units of chromatin. We show that histone tails, which are difficult to experimentally see because of their nature of being in intrinsically disordered, are a key to stabilize various conformations.第57回日本生物物理学会年