TRANSCRIPTIONAL REGULATION OF Il9r IN RADIATION-INDUCED MOUSE T-CELL LYMPHOMA CELL LINE

Interleukin-9 (IL-9) is a multifunctional cytokine secreted from TH2 cell. Besides its role during immune and inflammatory responses, its growth promoting and antiapoptotic activities on multiple transformed cells suggest a potential role in tumorigenesis. We previously reported the over-expression and activation of the receptor of IL-9 (IL-9R) in radiation-induced T-cell leukemia (TL) of mice. However, the transcriptional regulation of murine IL-9R gene (Il9r) is rarely reported in T-lineage cells. In this study we established an Il9r-highly-expressing TL cell line and detected the promoter region located at -410 ~ -487 bp upstream translation start codon. EMSA and ChIP assay suggested that chromatin associated with this region was highly acetylated and that TBP was bound in vivo.KTCC 2009 International Worksho