重粒子線誘発DNA 障害の特徴

In order to evaluate the effects of space radiation to germ line cells and embryos, we irradiated mouse ES cells and the sell survival and their development was examined. Mouse cultured and frozen ES cells (-80 degree in C) were irradiated by X-ray, Fe and C ion heavy particles. The cells were plated and numbers of colony were counted. The mouse ES cells were most sensitive to Fe ion particles at 0-1Gy of exposure and carbon gave moderate effect to ES cells. Interestingly, radiation at low dose of 0-1 Gy, by Fe and C particles damaged ES cells seriously, while X-ray did not We also examined phosphorylation of histone H2AX after irradiation of X-ray, Fe particles. The result showed that low doses of Fe, C particles induced phosphorylation of histone H2AX.平成２０年度放射線医学総合研究所重粒子線がん治療装置等共同利用研究成果発表