Dose and Particle Dependence of Fluorescent Dots in DNA Sheet Observed with Fluorescence Imaging of Oxidative Damage of Guanine Induced by Heavy Ion irradiation at TIARA

To confirm a contribution of the low LET region of heavy-ion track, we irradiated DNA sheet with argon or proton beam, visualize the penumbra region with the aid of an immunofluorescence method using an antibody against 8-OHdG, and examined numbers and areas of the fluorescence dots in the DNA sheet. The result indicated that OH radical induced DNA damage was significantly produced even at very high LET argon ion, but the diameter of fluorescent dot was different from Chatterjee and Schaefer estimated from their ion track model