Therapeutic cloning, whereby nuclear transfer (NT) is used to generate embryonic stem cells (ESCs) from blastocysts, has been demonstrated successfully in mice and cattle. However, if NT-ESCs have abnormalities, such as those associated with the offspring produced by reproductive cloning, their scientific and medical utilities might prove limited. To evaluate the characteristics of NT-ESCs, we established more than 150 NT-ESC lines from adult somatic cells of several mouse strains. Here, we show that these NT-ESCs were able to differentiate into all functional embryonic tissues in vivo. Moreover, they were identical to blastocyst-derived ESCs in terms of their expression of pluripotency markers in the presence of tissue-dependent differenti...
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2003.Includes bibliographi...
Cloning of mammals by nuclear transfer (NT) results in gestational or neonatal failure with at most ...
This chapter describes a detailed protocol for deriving mouse embryonic stem (ES) cell lines from bl...
Cloning animals by nuclear transfer (NT) has been successful in several mammalian species. In additi...
Cloning methods in mice are now well described and are becoming routine. However, the frequency at w...
The establishment of human ES cells has given tremendous input to the concept of stem cell-based the...
Nuclear transfer (NT) provides an opportunity for clonal amplification of a nuclear genome of intere...
In a mouse model nuclear transfer embryo-derived embryonic stem cell lines (ntESCs) of various genet...
Embryonic stem cells derived from nuclear transfer embryos (ntESCs) are particularly valuable for re...
SummaryCloning mammals by nuclear transfer (NT) remains inefficient. One fundamental question is whe...
Abstract In a mouse model nuclear transfer embryo-derived embryonic stem cell lines (ntESCs) of vari...
In a mouse model nuclear transfer embryo-derived embryonic stem cell lines (ntESCs) of various genet...
SummaryThe recent finding that reprogrammed human pluripotent stem cells can be derived by nuclear t...
AbstractPluripotent human stem cells isolated from early embryos represent a potentially unlimited s...
SummaryIt is well known that oocytes can reprogram differentiated cells, allowing animal cloning by ...
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2003.Includes bibliographi...
Cloning of mammals by nuclear transfer (NT) results in gestational or neonatal failure with at most ...
This chapter describes a detailed protocol for deriving mouse embryonic stem (ES) cell lines from bl...
Cloning animals by nuclear transfer (NT) has been successful in several mammalian species. In additi...
Cloning methods in mice are now well described and are becoming routine. However, the frequency at w...
The establishment of human ES cells has given tremendous input to the concept of stem cell-based the...
Nuclear transfer (NT) provides an opportunity for clonal amplification of a nuclear genome of intere...
In a mouse model nuclear transfer embryo-derived embryonic stem cell lines (ntESCs) of various genet...
Embryonic stem cells derived from nuclear transfer embryos (ntESCs) are particularly valuable for re...
SummaryCloning mammals by nuclear transfer (NT) remains inefficient. One fundamental question is whe...
Abstract In a mouse model nuclear transfer embryo-derived embryonic stem cell lines (ntESCs) of vari...
In a mouse model nuclear transfer embryo-derived embryonic stem cell lines (ntESCs) of various genet...
SummaryThe recent finding that reprogrammed human pluripotent stem cells can be derived by nuclear t...
AbstractPluripotent human stem cells isolated from early embryos represent a potentially unlimited s...
SummaryIt is well known that oocytes can reprogram differentiated cells, allowing animal cloning by ...
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2003.Includes bibliographi...
Cloning of mammals by nuclear transfer (NT) results in gestational or neonatal failure with at most ...
This chapter describes a detailed protocol for deriving mouse embryonic stem (ES) cell lines from bl...