Polymicrobial Agents and Antibiotic Profile of Urinary Tract Infections among Pregnant Women in Anambra State, Nigeria

Microbiological studies were carried out on midstream urine collected from 200 pregnant women(test) and 100 non pregnant women (control) attending antenatal clinics at General Hospital Onitsha, Enugwu-Ukwu and Chizoba Hospital Awada, Obosi. All the subjects were between 18-37years. The urine samples were cultured on to freshly prepared Eosine methylene blue (Oxoid), blood and MacConkey (Difco) agar plates and incubated at 37oc for 24hours. Urinalysis using Combi-9 urine test strip and microscopic examination of the deposit were done. Organisms were identified using cultural characteristics, Gram staining reactions and some biochemical tests. Antibiotic sensitivity tests were carried out on isolates using agar diffusion methods. The result showed a prevalent rate of UTI in pregnancy 23(11.5%) and control 10(10%). There was no significant difference among pregnant and non- pregnant women p>0.05. Echerichia coli were the predominant organism isolated 78.26%, Group B streptococcus, Klebsiella spp and Staphylococuus saprophyticus were isolated 1 (4.3%) each, Proteus mirabilis were isolated 2(8.7%). In the control group E. coli were isolated 8 (80%) while Proteus and klebsiella were isolated 1(10%) each. All organisms isolated were 100% sensitive to gentamicin and nalidixic acid and 100% resistant to ampicillin. Keywords: Pregnancy, urinary tract infection, polymicrobial agents antibiotic profil