Synergistic Effects of Probiotics and Autogenous Bacterin Against Inositol Negative Motile Salmonella Species

Salmonellosis, a disease caused by pathogenic strains of Salmonella, has been reported to have an essential degree in morbidity and mortality of humans (especially farmers) and poultry resulting in low productivity. This study was undertaken to evaluate the synergistic effects of probiotics and autogenous bacterin against inositol negative motile Salmonella species isolated from different poultry feed collected from Orlu-west Local Government Area of Imo State. A total of 40 representative feed samples were aseptically collected and screened for the presence of inositol negative motile Salmonella species using pour plate method. The colonies generated from the primary isolation were sub-cultured, characterized and identified using their colony description, morphological and biochemical characteristics. The pathogenic potentials of the organism in broiler chicks were investigated by challenging the chicks orally using 0.5ml of the inoculum (108cells/ml).The protective effects of locally prepared autogenous bacterin (B), commercially prepared probiotics (P) and autogenous bacterin plus commercially prepared probiotics (BP) were investigated using in vivo technique. The study revealed the presence of inositol negative motile Salmonella species in the studied samples. There significant (p<0.05) pathological features and lesions in the liver and spleen of the infected chicks. The mean plate counts were significantly (p<0.05) recorded more in the liver than spleen. The in vivo study showed that P, B and BP showed pronounced activity against the tested isolates of which BP proved to be more effective