Evaluation of BRCA1 Gene Mutations Frequency in Breast Cancer Patients

Breast cancer is the most frequent carcinoma in females. The present study aimed to shed light on the association between the breast cancer incidence and BRAC1 gene mutations. This study was carried out on two groups of 30breast cancer patients and 10 of healthy control. Polymerase chain reaction (PCR) for exon 2 of BRCA1 was performed to detect the gene mutations.The results showed 21(70%) samples were negative to BRCA1gene mutation while 9 (30%) samples were positive to it, which was a significant difference (p value=0.0984 <0.05). The statistical analysis showed no significant differences in the frequency of BRCA1 gene mutation with age and family history. In correlation with the tumor stage, the highest percentage of BRCA1gene mutation positive samples 7(77.77%) were stage II that statistically no significant differences from percentage of stage III.In conclusion, the BRCA1 gene mutation may play a key role in breast cancer incidence and have a relatively high frequency in tumor stage II . Keywords: Breast cancer, BRCA1 Gene ,mutatio