Pseudomonas aeruginosa: Antibiotic resistance pattern to different isolates in Al-Hillah city, Iraq

Aim: Pseudomonas aeruginosa (Ps. aeruginosa) considered as most important bacteria which can isolated from various kinds of infection. This study tries to survey the infections caused by Ps. aeruginosa especially medical and surgical care units and try to reveal the antimicrobial agents susceptibility against Ps. aeruginosa. Material and Method: This study was conducted during September 2012 to February 2013. During this period total of 285 samples were tested and showed growth of bacteria. The isolates of Pseudomonas aeruginosa were selected on the basis of their growth on Nutrient agar pigmented and non-pigmented colonies with oxidase positive and on routine MacConkey medium which showed lactose Non-fermenting pale colonies. Antimicrobial susceptibility of all the isolates was performed using disc-diffusion (Modified-Kirby Baur method) according to CLSIs guidelines. Result: In present study, maximum isolates of Ps. aeruginosa isolated from various samples. The isolates were obtained from different clinical specimens, including pus, urine, respiratory fluids, blood, tissue, and genitalia. All the clinically isolated samples were identified as P. aeruginosa. Out of 285, 74.04% are males and 25.96% are females. Most of patients were aged between 27-48 years. Approximately half the isolates tested were from community patients, mostly from infections of the Wound/Pus (22.46%), urinary tract (22.11%), Swab (18.6%) and Respiratory Tract (15.09%). P. aeruginosa strains screened showed sensitivity to AK \Amikacin, E \Erythromycin and P\Penicillin while showed resistance to penicillin, erythromycin, and norfloxacin, AX \Amoxicillin, AMC \Amoxicillin + Clavulanic acid, AZM \Azithromycin. Conclusion: To prevent the spread of the resistant bacteria, it is critically important to have strict antibiotic policies while surveillance programs for multidrug resistant organisms and infection control procedures need to be implemented