Aggregate Image Analysis Code

These FIJI ImageJ macros were used to determine volumes of aggregates of particles in z-stacks. "Aggregate_Counting_Macro_1.2.4_for_publication.ijm" takes in a z-stack and generates various statistics about aggregates including volume, surface area, and position. Results for Test_dataset_08_01_18 are located in the Results folder. "Average_Singlet_Size_Macro_1.0.0_for_publication.ijm" takes the object map output from the Aggregate_Counting_Macro and prompts the user to record the ID number associated with 10 single particles. The singlets can then be used to convert aggregate volumes from the Aggregate_Counting_Macro into particle counts. Written in the ImageJ macro language. Test dataset was run in FIJI version 2.00-rc-68/1.52e, Build:bad6864e55 using macOS Version 10.13.4