Data from: Shotgun mitogenomics across body size classes in a local assemblage of tropical Diptera: phylogeny, species diversity and mitochondrial abundance spectrum
- Choo, Le Qin
- Crampton-Platt, Alex
- Vogler, Alfried P.
DOIAbstract
Mitochondrial genomes can be assembled readily from shotgun-sequenced DNA mixtures of mass-trapped arthropods (“mitochondrial metagenomics”), speeding up the taxonomic characterization. Bulk sequencing was conducted on some 800 individuals of Diptera obtained by canopy fogging of a single tree in Borneo dominated by small (<1.5 mm) individuals. Specimens were split into five body size classes for DNA extraction, to equalize read numbers across specimens and to study how body size, a key ecological trait, interacts with species and phylogenetic diversity. Genome assembly produced 304 orthologous mitochondrial contigs presumed to each represent a different species. The small-bodied fraction was the by far most species-rich (187 contigs). Iden...
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