Potential Protective Effects of Caffeic Acid Phenethyl Ester (CAPE) Against H202-Induced Oxidative Stress in Cardiac H9c2 Cells Compared To Common Antioxidants

Caffeic Acid Phenethyl Ester (CAPE) is a natural compound that has previously exhibited anti-proliferative, anti-inflammation and antioxidant activities. However, CAPE\u27s effects have not been fully elucidated in myoblasts under oxidative stress. We compared CAPE\u27s cytoprotective effects to several known antioxidants (caffeic acid, vitamin C, and trolox) in H9c2 cells following oxidative injury by hydrogen peroxide (H202). H9c2 cells incubated with H202 treatment ( 100-700 μM, n=4) for 24 hours dosedependently reduced cell viability (assessed by a cell counting assay). Compared to a reduction in cell viability from H202 500 μM treatment (22 ± 4% of control), H9c2 cells were significantly restored by pretreatment of CAPE at 10 μM (100 ± 25%), 20 μM (112 ± 15%) and 40 μM (109 ± 15%, n=5,