Isolated cell nuclei were used as the source of template DNA to investigate the role of a cytosolic aprotinin-binding protein (ADR) in the initiation of eukaryotic DNA replication. Computerized image cytometry demonstrated that the DNA content of individual nuclei increased significantly following incubation with ADR-containing preparations, and the extent of DNA synthesis is consistent with that allowed by the limiting concentration of dTTP. Thus, dTTP incorporation into isolated nuclei represents DNA synthesis and not parent strand repair. We found that dTTP incorporation into the isolated nuclei is dependent on DNA polymerase a (a principal polymerase in DNA replication) but that DNA polymerase ß (a principal polymerase in DNA repair pr...
The precise coordination of the different steps of DNA replication is critical for the maintenance o...
Precise DNA replication is essential for the maintenance of a healthy genome. Although DNA replicati...
It has been shown that DNA primase activity is tightly associated with 10S DNA polymerase a from cal...
We previously investigated the role of an aprotinin-binding protein (ADB) in the initiation of DNA r...
Cytoplasmic extracts prepared from T cell lines undergoing antigen-specific, interleukin-2 (IL-2)-de...
Proliferating lymphocytes contain an intracellular factor, ADR (activator of DNA replication), which...
The contributions of human DNA polymerases (pols) alpha, delta and epsilon during S-phase progressio...
The initiation of DNA replication in T lymphocytes appears to be regulated by two distinct activitie...
Assessing DNA repair is an important endpoint to study the DNA damage response for investigating the...
DNA synthesis in isolated nuclei from Hela cells has been investigated. The cells were synchronized ...
In mammalian cells, both semiconservative DNA replication and the DNA repair patch synthesis Induced...
Eukaryotic DNA polymerase delta and its accessory proteins are essential for SV40 DNA replication in...
In order for cells to divide, they must replicate their chromosomes precisely once. This replication...
To evaluate the relative contributions of DNA polymerase <y and DNA polymerase 8 in chromosome re...
The precise coordination of the different steps of DNA replication is critical for the maintenance o...
The precise coordination of the different steps of DNA replication is critical for the maintenance o...
Precise DNA replication is essential for the maintenance of a healthy genome. Although DNA replicati...
It has been shown that DNA primase activity is tightly associated with 10S DNA polymerase a from cal...
We previously investigated the role of an aprotinin-binding protein (ADB) in the initiation of DNA r...
Cytoplasmic extracts prepared from T cell lines undergoing antigen-specific, interleukin-2 (IL-2)-de...
Proliferating lymphocytes contain an intracellular factor, ADR (activator of DNA replication), which...
The contributions of human DNA polymerases (pols) alpha, delta and epsilon during S-phase progressio...
The initiation of DNA replication in T lymphocytes appears to be regulated by two distinct activitie...
Assessing DNA repair is an important endpoint to study the DNA damage response for investigating the...
DNA synthesis in isolated nuclei from Hela cells has been investigated. The cells were synchronized ...
In mammalian cells, both semiconservative DNA replication and the DNA repair patch synthesis Induced...
Eukaryotic DNA polymerase delta and its accessory proteins are essential for SV40 DNA replication in...
In order for cells to divide, they must replicate their chromosomes precisely once. This replication...
To evaluate the relative contributions of DNA polymerase <y and DNA polymerase 8 in chromosome re...
The precise coordination of the different steps of DNA replication is critical for the maintenance o...
The precise coordination of the different steps of DNA replication is critical for the maintenance o...
Precise DNA replication is essential for the maintenance of a healthy genome. Although DNA replicati...
It has been shown that DNA primase activity is tightly associated with 10S DNA polymerase a from cal...