The Role of DONs in Regulating Bone Tumor Formation from Mesenchymal Stem Cells

Osteosarcoma is a differentiation disease that results from an osteoblast differentiation and proliferation imbalance. This study investigated how certain 19-base short regulatory RNAs may disrupt BMP9-induced osteoblast differentiation in mesenchymal stem cells (MSCs). We are interested in determining these effects because they may further explain the development of osteosarcoma. We introduced a completely randomized 19-base short RNA Library into MSCs and discovered several short RNA transcripts, which are named as Disruptors of BMP9 (DONs). We showed that these DONs can produce osteogenic resistant MSCs at various time points during library screening. We found that MSCs affected with DONs produce a bone tumor-like phenotype. This study determined that iMAd 199 mesenchymal stem cells stably expressing either DONs 1, 2, and 3 effectively disrupted BMP9-induced osteogenic differentiation. Based on these results, we hope to sequence the DONs’s RNA to determine any up or down regulation of mRNA or lncRNA involved in the pathogenesis of osteosarcoma