Rp-HPLC Method for Simultaneous Estimation of Levocetirizine Dihydrochloride and Montelukast Sodium in tablets

A simple, selective and sensitive reverse phase high performance liquid chromatography (Rp-HPLC) method has been proposed for the simultaneous quantitative determination of levocetirizine dihydrochloride (LEV) and Montelukast sodium (MON) in pure form as well as in its pharmaceutical formulation. The chromatography was carried out on Waters C18 analytical column (15cm × 4.6 mm, 5µ) using a mobile phase of methanol: water (75:25 v/v). The flow rate was 1.0 ml/min with detection at 235 nm. The retention time of LEV and MON were found to 2.88 and 3.83 min respectively. The linearity for LEV and MON were in the range of 50-150 µg/mL and 100- 300 µg/mL respectively. The recoveries of LEV and MON were found to be 100.00% and 99.00%, respectively. The proposed method was validated and successfully applied to the estimation of LEV and MON in combined tablet dosage forms