高リン食で飼育したラットの腎無細胞画分のCa,Mg,P量と脂肪酸組成

We studied the alteration of kidney function with increase kidney calcium, magnesium and phosphorus contents in rats fed a high-phosphorus diet. Five-week-old male SD rats were used. The rats were fed a diet with either a control (0.3%) or a high (1.5%) level of phosphorus for 20 days. Body weight gain and food intake were depressed, and nephrocalcinosis was determined by analysis of kidney calcium in rats fed a 1.5% phosphorus diet. Calcium, magnesium and phosphorus contents of nucleus fraction, mitochondrial fraction, microsomal fraction and cytosol of kidney were determined. And composition of fatty acid and thiobarbituric acid reactive substances (TBARS)in each fraction were measured. In every fraction from rats fed a 1.5% phosphorus diet, contents of calcium, magnesium and phosphorus were significantly elevated than those of a control diet. Composition of fatty acid of mitochondrial fraction and microsomal fraction in rats fed a 1.5% phosphorus diet were different from those of a control diet. But TBARS in the mitochondrial and microsomal fractions were same