Producing Monoclonal Antibodies To Phospholipase A2

Monoclonal antibodies were produced to phospholipase A2, a protein allergen of honey bee venom. The hybridoma technique, which involves fusing antibody-producing spleen cells to myeloma cells was used. The antibodies were of the IgG1 subclass and they did not cross-react with phospholipase components of other bee venoms