MONITORING OCCURRENCE OF NEMATODE VECTOR VIRUS FROM GENUES XIPHINEMA IN VINEYARDS OF VUKOVAR-SRIJEM, OSIJEK-BARANJA AND ISTRIAN COUNTIES, 2018.

Nematode roda Xiphinema su jedna od vrlo važnih grupa organizama u tlu koje su prilagođene životu u različitim uvjetima. Hrane se na korijenu vinove loze a neki su prenosioci virusa lepezastog lista vinove loze (GFLV). Cilj ovog istraživanja bio je praćenje pojave nematoda prenosioca virusa iz roda Xiphinema u vinogradima Vukovarsko-srijemske, Osječko-baranjske i Istarske županije u 2018. godini. Uzorci su uzeti sa 13 lokalite unutar 3 županije. (Vukovarsko-srijemsku, Istarsku i Osječko-baranjsku). Uzorak se uzimao u zoni korijena na dubini od 30 cm te unutar 24-48 sati dostavio na analizu u Laboratorij za nematologiju na Fakultetu agrobiotehničkih znanosti Osijek. Izdvajanje nematoda iz uzorka tla ispiranjem vršilo se metodom Baermannovih lijevaka. Nakon što su nematode izdvojenje iz tla slijedi priprema trajnog preparata kako bi se nematode moglo promatrati na mikroskopu te izmjeriti njihove tjelesne karakteristike. Sve nematode koje su determinirane iz uzoraka pripadale su vrsti Xiphinema index, a nalazile su se u Vukovarsko-srijemskoj i Istarskoj županiji. Ovim istraživanjem pokazalo se kako je Xiphinema index prisutna nematoda u hrvatskim vinogradimaNematode Xiphinema is one of the most important groups of organisms in the soil that are adapted to life under different conditions. They are feeding on the roots of vines and some of them are vectors of Grapevine fanleaf virus (GFLV). The goal of this study was to monitor the occurrence of nematode virus transmitters from the genues Xiphinema in vineyards of Vukovar-srijem, Osijek-baranja and Istrian counties in year 2018. Samples were taken from 13 localities within 3 county (Vukovarsko-srijemsku, Istarsku i Osječko-baranjsku). They were taken from the root zone at a depth of 30 cm and delivered within 24-48 hours in the Nematology Laboratory at the Faculty of agrobiotechnical Sciences Osijek. The extraction of the nematode from the soil sample was done using the Baermann funnel method. Once the nematodes are extracted from the soil, the making of the permanent preparation is followed to allow the nematodes to be observed on the microscope and to measure their physical characteristics. All nematodes that were determined from the samples belonged to the Xiphinema index variety and they were found in Vukovar-srijem and Istrian county. This study showed that Xiphiema index is present nematodes in Croatian vineyards