Effect of obestation and treadmill exercise on lipid profile and serum malondialdehyde (MDA) levels in rat model

Objective: The present study investigates the effect of intraperitoneally (IP)-administrated obestatin and short-term treadmill exercise on serum malondialdehyde (MDA) and lipid profile in rat model. Materials and Methods: Forty-five rats weighing 100 ± 5g were selected and divided into five experimental groups; Group-1/control was normally kept and did not receive obestatin-included solution or exercise. Group-2 (placebo) was treated with basal solution (50 ml distilled water). Group-3 was referred to as obestatin-group which was subjected to injection during the experiment with obestatin solution (10 μg obestatin/ rat). Group-4 was known as treadmill-group which was subjected to exercise protocol (15 days treadmill training), and group-5, known as obestatin and treadmill group (OT), was subjected to injection with obestatin and exercise protocol. The exercise-groups were trained for 3-weeks. Results: the treadmill-group was decreased total cholesterol (TC) concentration (67.67 mg/dl), in comparison with control and obestatin-group (79.00 and 78.33 mg/dl, respectively). Similarly with TC level, the triglyceride and HDL cholesterol (HDL-C) was decreased in treadmill-group (‌triglyceride: 70.66 mg/dl in compared with 87.67 mg/dl in control, and HDL-C: 46.10 mg/dl in compared with 57.33 mg/dl in control; P<0.05). Also, level of MDA was decreased in treadmill- and treadmill-obestatin groups. Conclusion: short-term treadmill exercise significantly reduced plasma lipid profile and MDA levels in rat model. Nevertheless, administration of obestatin had no significant effect on lipid profile and MDA. Furthermore, obestatin-administration in combination with treadmill-exercise did not have synergic effect on plasma lipids, MDA level and total protein in rats.Objetivo: O presente estudo investiga o efeito da obestatina administrada por via intraperitoneal (IP), exercício em esteira de curta duração no malondialdeído sérico (MDA) e no perfil lipídico no modelo de ratos. Materiais e Métodos: Quarenta e cinco ratos pesando 100 ± 5g foram selecionados e divididos em cinco grupos experimentais; O grupo 1 / controle foi normalmente mantido e não recebeu solução ou exercício incluído na obestatina. O grupo 2 (placebo) foi tratado com solução basal (50 ml de água destilada). O grupo 3 foi referido como grupo de obestatina, que foi submetido a injeção durante o experimento com solução de obestatina (10 μg de obestatina / rato). O grupo 4 era conhecido como grupo de esteira que foi submetido ao protocolo de exercícios (treinamento de 15 dias em esteira) e o grupo 5, conhecido como grupo de obestatina e esteira (OT), foi submetido à injeção de obestatina e protocolo de exercício. Os grupos de exercícios foram treinados por 3 semanas. Resultados: o grupo da esteira apresentou diminuição da concentração de colesterol total (CT) (67,67 mg / dl), em comparação com o grupo controle e o grupo da obestatina (79,00 e 78,33 mg / dl, respectivamente). Da mesma forma que com o nível de CT, o triglicerídeo e o HDL-colesterol (HDL-C) diminuíram no grupo da esteira (triglicerídeo: 70,66 mg / dl em comparação com 87,67 mg / dl no controle e HDL-C: 46,10 mg / dl em comparação com 57,33 mg / dl no controle; P