Induction of synchronous estrus in Morada Nova ewes: effects of distinct progestagen duration regimen and non-surgical embryo recovery as a tool to access efficiency measurements.

Abstract: The first stet for applying superovulation is to know the responsiveness of the ewe to different estrous synchronous protocol duration and possible implications on embryo yield. On the other hand for nonsurgical embryo recovery (NSER) a well consolidated protocol should be tested and, in Morada Nova ewes, successful non-surgical uterine access was reported (Fonseca et al., 2019. Reprod Fertil Devel, 31:17-26). In this context, the objective of this study was to evaluate the efficiency different synchronous estrus induction and NSER as additional tool to evaluate the results these protocols in pluriparous Morada Nova ewes. A total of 18 pluriparous sheep were divided into three treatment groups with 6 (T6, n=6), 9 (T9, n=6) and 12 (T12, n=6) days of 60 mg of medroxyprogesterone acetate (Progespon®, Zoetis, Campinas, São Paulo, Brazil). The NSER mean duration was 27.6±1.4 min, with recovery of 99.8% of injected fluid and embryo recovery rate of 62.97% (17/27). These preliminary results indicated that all protocols provided similar results and that NSER can be successfully performed and used as additional toll in synchronous estrous induced Morada Nova ewes independently from the duration of progestogen regimen to assure that viable embryos can be produced by ewes subjected by these protocols. [Indução do estro sincrônico em ovelhas Morada Nova: efeitos do regime de duração distinto do progestágeno e recuperação não cirúrgica do embrião como uma ferramenta para acessar medidas de eficiência].Edição dos resumos do XXIII Congresso Brasileiro de Reprodução Animal, Gramado, RS, Brasil, 15 a 17 de maio 2019