Developing an Intracellular Neurobiology Lab Sequence for Use in an Interdisciplinary Neurobiology Course

The goal of my research was to evaluate procedures currently used in the Intracellular Leech Lab in order to modify the Neurobiology ZOOL475 Lab to increase the rate of successful intracellular recordings for future Neurobiology students. Two approaches were used: the first was to evaluate the possibility of using a less expensive leech species in order to allow students unlimited attempts at recording from ganglia without budget constraints. The second was to evaluate various raising conditions (water, temperature, and age) to optimize the quality of the leech ganglia. Both approaches provided useful information to improve lab success