The Effects of Silver Nanoparticles on Mouse Embryonic Cell Renewal and Cell Cycle

The use of silver nanoparticles in commercially made products is rapidly increasing, and there is no regulation on the disposal of these nanoparticles. As human exposure to silver nanoparticles rises, this study determines the effects of this exposure on stem cell factor gene expression and stem cell fate. This was accomplished by introducing varying concentrations of silver nanoparticles into mouse embryonic stem cells for varying amounts of time. Western blot and immunoprecipitation techniques were run on these cells to determine how the responses of stem cell factors Oct4, Nanog, P53, SirT1, and Rb differ from their normal function within the cell. In addition, this study also determines whether programmed cell death is occurring in response to the silver nanoparticle treatment. The results of the research provided necessary scientific data to improve or eliminate potential toxicity of nanoparticles, and information for relevant authority when approving products for consumer uses.https://ecommons.udayton.edu/stander_posters/1166/thumbnail.jp