Defining the role of two newly identified proteins in the Vibrio vulnificus TonB2 system

Vibrio vulnificus is a human pathogenic bacteria that is closely related to Vibrio cholera, the disease causing agent of cholera. Three TonB transport systems are found in the outer membrane of V. vulnificus and are responsible for powering the uptake of iron bound siderophores into the cell. Within the Ton82 and TonB3 systems are two proteins, Orf1 and Orf6. The function and necessity of Orf1 and Orf6 to the TonB systems were investigated through the use of growth, motility, and antibiotic sensitivity assays and RNA transcriptional level analysis