Binding and Structural Effects of Argyrin B on E. coli derived EFG

Bacterial antibiotic resistance is a preeminent threat to the heath and welfare of humanity. This work aims to add mechanistic understanding of antibiotic resistance by examining the effects of argyrin B on a fundamentally conserved translation factor and GTPase, elongation factor-G. EF-G is a crucial facilitator in bacteria successfully synthesizing proteins. By examining the binding and structural effects of argyrin B on an E. coli derived EF-G we hope to elucidate the mechanism of this antibiotic and its overall effect on the GTPase. We would accomplish this using various assays to assess binding effects, enzyme activity, and corroborate these effects with structural data. Learning the mechanism of action of argyrin B could allow research to progress further on these peptide derived drug candidates, aiding in the fight against antibiotic resistance. (GTPase: Guanodine Triphosphate-ase