Separation of proteins via continuous pH parametric pumping

This dissertation covers the separation of proteins via recuperative mode pH parametric pumping. Continuous pH parametric pumping separations using the model system haemoglobin and albumin on a CM-Sepharose cation exchanger have been experimentally and theoretically investigated. The pH parametric pumping system has a feed containing the protein mixture to be separated, introduced alternately to the top and bottom of the column. The top and bottom products are withdrawn from the apparatus during the bottom and top feed, respectively. Both equilibrium and nonequilibrium cases were considered, and theoretical results agreed with the experimental data. Buffer concentration, flow rate, reservoir displacement, and feed volumes have been found to be important in determining the pH parametric pumping performance. Many proteins are processed batchwise. Parametric pumping as described here offers the possibility of continuous processing, It is shown that under certain conditions, the pH parametric pumping is capable of achieving high separation. Moreover, the continuous process can be operated with a larger feed throughput