EF FE CT O F T OM AT O J UIC E (L y co p ers ic u m esculentum) ON SPERMATOGENIC CELLS AND LEYDIG CELLS IN MICE (Mus musculus)INDUCEDBY MONOSODIUM GLUTAMATE

The aim of this research was to discover effect of tomato juice (Lycopersicum esculentum) on spermatogenic cells and leydig cells in mice (Mus musculus) induced by monosodium glutamate. This research used twenty five male white mice aged 3-5 months with a weight around 20 grams. This research consisted of five groups with each different treatment. Control group was the control treatment, T0, T1, T2, and T3, were given with MSG with dose 4000mg/kgBW and tomato juice with each concentration of 25%, 50%, and 100%. Treatments had been given per oral for 49 days, once a day. At the end of the treatment period, execution was carried out, then the histopathological examination was performed. Microscopic observation on the spermatogenic cells and leydig cells showed that the T0 has not significantly difference with T1. Meanwhile T0 has significantly difference with control, T2, and T3. In additional. It shows that given MSG decrease number of spermatogenic cells and leydig cells. The decreasing number of spermatogenic cells and leydig cells caused by oxidative stress. Meanwhile oxidative stress increased reactive oxygen species. Reactive oxygen species have been implicated in cell damage and necrosis due to their direct oxidizing effects. Tomatoes contain lots of lycopene which is a group of carotenoids such as beta-carotene. Lycopene act as antioxidant that prevent oxidizing effect from MSG