THE EFFECT OF ADDITION GREEN TEA LEAF (Camellia sinensis) EXTRACT IN EGG YOLK SKIM MILK EXTENDER ON POST-THAWED MERINO SHEEP SPERM QUALITY

The purpose of this research was to determined the best dosage of green tea leaf extract in egg yolk skim milk extender for post thawed Merino sheep sperm quality that measured by motility, viability, membrane integrity and the level of malondialdehyde post thawed. The treatment was divided into four groups: egg yolk and skim milk diluter, 0.05 mg green tea leaf extract in 100 ml egg yolk skim milk extender, 0.10 mg green tea leaf extract in 100 ml egg yolk skim milk extender and 0.15 mg green tea leaf extract in 100 ml egg yolk skim milk extender. The sperm quality was observed post thawing. The data were analyzed by SPSS One Way Analysis of Variant (ANOVA) and Duncan test. The result showed that the best sperm quality was T1,motility 43.80%,viability 55.84%, membrane integrity 36.90% and MDA level 7762.50nmol/ml with addition 0.05 mg green tea leaf extract. The conclusion of this research is addition of green tea leaf extract in egg yolk skim milk extender increasedthe quality of Merino sheep sperm post thawed