Identification Of Shiga Toxin-Producing Esherichia coli In Raw Milk Samples From Dairy Cows In Surabaya, Indonesia

The purpose of this research was to identify the presence of shiga toxinproducing Escherichia coli (STEC) in raw milk samples in Surabaya dairy cows using Multiplex Polymerase Chain Reaction (MPCR) assay. Approximately 10 ml milk samples from 75 apparently healthy Holstein Friesian cows from Surabaya, Indonesia were analyzed. Milk samples were inoculated with brilliant green bile broth (BGBB), subcultured in eosin methylene blue agar (EMBA) and were confrmed biochemically using Indol test. Multiplex PCR using primer flicH7 and primer stx2 (gene coding shiga toxin) were then performed. Results showed that 26 out of 75 samples were E. coli in bacterial isolation and MPCR. Moreover, two samples (7.7%) were positive for stx2 gene. The MPCR assay described in the present study can be employed to identify and screen for E. coli harboring stx2 gene in raw milk from dairy cows in Indonesia. Keywords: Escherichia coli, multiplex PCR, shiga toxin, stx2 gen