Optimization of protein yields in water extracts of Jackiopsis ornata roots by response surface methodology using microwave assisted extraction (MAE)

Optimization of protein yields in water extracts that were obtained from Jackiopsis ornataroots by Microwave assisted extraction (MAE) was established by a Circumscribed Central Composite Design (CCCD). MAE proved to be an optimum choice for the protein extraction. The highest yield (20.425±108) was obtained with the parameters liquid to solid ratio (S= 30:1), particle size (P=0.022±0.002mm (radius), temperature (T= 65°C); microwave power (W= 300W) and extraction time (t= 20 minutes). Statistical analysis illustrated the adequacy of the generated full quadratic equation with ad high values of the coefficient of determination (R2), adjusted coefficient of determination R2adj and predicted coefficient p of determination R2pred The p value of the Lack of Fit test illustrated the adequacy of the model. The optimization conditions of extracting the protein was predicted to be with the parameters S: 26, P: 0.0284; T: 65 °C; P: 303 W and t: 20 min. The high efficiency of MAE to obtain J. ornataprotein extract encouraged further application on pilot and industrial scale