Multispectral techniques applied to photomicrography

Based upon an objects absorbtion and reflection characteristics to varying amounts of radiation, a series of selective wavelength band exposures were made for color separation of a microscopic specimen. The exposures axe made to record the specimens characteristic transmission or reflection patterns as a visible density value. The exposeures were made in a overlapping band width manner. The exposures were in the blue, green, and red regions of the spectrum. The resultant negetives were printed on Polycontrast paper using filter No. 2. The main purpose of the experiment was the design of a photographic system to remove the biological step of staining. This was through the use of the spectral transmission curves of the componet parts of the specimen. The results of the separations must than be referenced to the spectral output of the specimens componet parts. This was not tried because of the region of the spectrum that was needed was in the far ultraviolet