Characterization of monoclonal antibodies against digoxin

Five antibodies directed against digoxin were grown and characterized. Two separation techniques were tested to find the better method of separating bound the free fractions of ligand after competitive reactions with the antibodies. Charcoal separation was chosen. The antibodies were analyzed for affinity constant by the Scatchard Analysis. Cross reactivity data for two of the antibodies were collected. Four of the antibodies have satisfactory affinity constants for use in a digoxin assay. Cross reactivity studies showed varying amounts of cross reactivity with digoxin metabolites, but none with the steroids and lipid tested