Expression of the multimeric and highly immunogenic brucella spp. Lumazine synthase fused to bovine rotavirus VP8D as a scaffold for antigen production in tobacco chloroplasts
- Alfano, Edgardo Federico
- Lentz, Ezequiel Matias
- Bellido, Demian
- Dus Santos, María José
- Goldbaum, Fernando Alberto
- Wigdorovitz, Andrés
- Bravo Almonacid, Fernando Felix
DOIAbstract
Lumazine synthase from Brucella spp. (BLS) is a highly immunogenic decameric protein which can accommodate foreign polypeptides or protein domains fused to its N-termini, markedly increasing their immunogenicity. The inner core domain (VP8d) of VP8 spike protein from bovine rotavirus is responsible for viral adhesion to sialic acid residues and infection. It also displays neutralizing epitopes, making it a good candidate for vaccination. In this work, the BLS scaffold was assessed for the first time in plants for recombinant vaccine development by N-terminally fusing BLS to VP8d and expressing the resulting fusion (BLSVP8d) in tobacco chloroplasts. Transplastomic plants were obtained and characterized by Southern, northern and western blot....
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