Increased glucose metabolic activity is associated with CD4+ T-cell activation and depletion during chronic HIV infection

Objectives: Glucose metabolism plays a fundamental role in supporting the growth, proliferation and effector functions of T cells. We investigated the impact of HIV infection on key processes that regulate glucose uptake and metabolism in primary CD4+ and CD8+ T cells. Design and methods: Thirty-eight HIV-infected treatment-naive, 35 HIV+/ combination antiretroviral therapy, seven HIV+ long-term nonprogressors and 25 HIV control individuals were studied. Basal markers of glycolysis [e.g. glucose transporter-1 (Glut1) expression, glucose uptake, intracellular glucose-6-phosphate, and L-lactate] were measured in T cells. The cellular markers of immune activation, CD38 and HLADR, were measured by flow cytometry. Results: The surface expression of the Glut1 is up-regulated in CD4+ T cells in HIVinfected patients compared with uninfected controls. The percentage of circulating CD4+Glut1+ T cells was significantly increased in HIV-infected patients and was not restored to normal levels following combination antiretroviral therapy. Basal markers of glycolysis were significantly higher in CD4+Glut1+ T cells compared to CD4+Glut1- T cells. The proportion of CD4+Glut1+ T cells correlated positively with the expression of the cellular activation marker, HLA-DR, on total CD4+ T cells, but inversely with the absolute CD4+ T-cell count irrespective of HIV treatment status. Conclusion: Our data suggest that Glut1 is a potentially novel and functional marker of CD4+ T-cell activation during HIV infection. In addition, Glut1 expression on CD4+ T cells may be exploited as a prognostic marker for CD4+ T-cell loss during HIV disease progression.Fil: Palmer, Clovis S.. Burnet Institute; AustraliaFil: Ostrowski, Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; ArgentinaFil: Gouillou, Maelenn. Burnet Institute; AustraliaFil: Tsai, Louis. Monash University; AustraliaFil: Yu, Di. Monash University; AustraliaFil: Zhou, Jingling. Burnet Institute; AustraliaFil: Henstridge, Darren C.. Baker IDI Heart and Diabetes Institute; AustraliaFil: Maisa, Anna. Burnet Institute; AustraliaFil: Hearps, Anna C.. Burnet Institute; Australia. Monash University; AustraliaFil: Lewin, Sharon R.. Burnet Institute; Australia. Monash University; Australia. The Alfred Hospital; AustraliaFil: Landay, Alan. Rush University Medical Center; Estados UnidosFil: Jaworowski, Anthony. Burnet Institute; Australia. Monash University; AustraliaFil: McCune, Joseph M.. University of California; Estados UnidosFil: Crowe, Suzanne M.. Burnet Institute; Australia. Monash University; Australia. The Alfred Hospital; Australi